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Resolution: standard / high Figure 3.
Positive ion mode electrospray mass spectrum of the refolded, full-length, mature AprA. The major peak in the spectrum (49 500 Da) corresponds exactly to the recombinant protein without the initiator methionine. The peak at 49 534 Da may represent a calcium adduct. The peak at 49 632 Da corresponds to AprA retaining the N-terminal methionine.
Walasek and Honek BMC Biochemistry 2005 6:21 doi:10.1186/1471-2091-6-21 |