Figure 3.

Identification of the 17α-HSD activity by TLC. A- Incubation of [14C]-4-dione with cells stably expressing mouse 17β-HSD type 5 (1) and 17α-HSD (2) activity. Standards of 4-dione (3), T (4) and epiT (5) have been deposited and co-migrated. B- Incubation of [14C]-ADT with cells cells stably expressing mouse 17β-HSD type 5 (1) and 17α-HSD (2) activity. Standards of ADT (3), 3α-diol (4) and epi3α-diol (5) have been deposited and co-migrated.

Bellemare et al. BMC Biochemistry 2005 6:12   doi:10.1186/1471-2091-6-12
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