Table 2

Predicted binding energy of each mutant subunit calculated using AMBER89 and CHARMM22 force fields.


Binding energy (kcal/mol)



AMBER89
CHARMM22

Wild type
-249
-118
Asn340Phe/Tyr418Phe
-291
-134
Asn340Phe/Tyr418Ile
-273
-126
Thr416Val/Thr417Val
-278
-131

Tanaka et al. BMC Biochemistry 2005 6:1   doi:10.1186/1471-2091-6-1