Expression analysis of the DR0975 gene in cultures exposed todifferent conditions. The level of DR0975 mRNA expressed in logarithmic (log) and stationary (sta) phase cells, in cells exposed to hydrogen peroxide (per) or menadione (sup) and from cells subjected to rapid desiccation by freeze drying (rap) or slow (slo) dehydration, followed by rehydration in each case, was quantified by RT-PCR as described in Materials and Methods. Each value represents the mean of triplicate amplifications of each of three independently prepared RNA samples for each condition. The amount of amplified product was determined by densitometry by comparison with the concentrations of the standards amplified from genomic DNA by gene-specific primers. The limit of detection was 4.23 × 10-16 M.
Fisher et al. BMC Biochemistry 2004 5:7 doi:10.1186/1471-2091-5-7