Protein Concentration Dependence to R. capsulatus PBGS Specific Activity. Protein was varied from 0.01 μg ml-1 to 40 μg ml-1 and assay times used were 5 min (○) and 1 h (□) with the purified R. capsulatus enzyme. The solid line represents a hyperbolic fit to the activity data presented for the combined 5 min and 1 h assays. The final pH for the R. capsulatus assays was 8.3 in BTP-HCI. For comparison, protein concentration curves are presented from previous experiments for B. japonicum PBGS (+)  and P. sativum PBGS (Δ,)  and the hyperbolic fits are presented as dotted and dashed lines respectively.
Bollivar et al. BMC Biochemistry 2004 5:17 doi:10.1186/1471-2091-5-17