|
Percentage distribution of the marker enzymes |
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| Enzymes |
Percentage Distribution (%) |
|||
|
|
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| B |
D |
E |
F |
|
|
|
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| Peroxisomal markers |
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| Catalase |
23.0 (0.3) |
34.2 (0.2) |
4.6 (0.8) |
38.2 (0.2) |
| Urate oxidase |
14.2 (1.0) |
28.7 (0.4) |
6.0 (0.7) |
51.1 (0.2) |
| D-aminoacid oxidase |
21.2 (0.5) |
30.5 (0.2) |
4.2 (1.0) |
44.1 (0.3) |
| Fatty acyl-CoA oxidase |
7.1 (0.7) |
22.7 (0.3) |
3.7 (0.6) |
66.5 (0.2) |
| Glycolate oxidase |
11.6 (0.7) |
27.3 (0.2) |
7.7 (0.6) |
53.5 (0.2) |
| Mitochondrial marker |
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| Succinate dehydrogenase |
56.0 (0.1) |
40.4 (0.2) |
1.9 (0.5) |
2.2 (0.8) |
| Lysosomal marker |
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| Aryl sulphatase |
37.4 (0.4) |
30.5 (0.3) |
7.9 (0.7) |
24.4 (0.2) |
| Microsomal marker |
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| NADPH cytochrome c reductase |
15.5 (0.6) |
18.2 (0.5) |
38.5 (0.4) |
27.9 (0.5) |
| Protein |
12.9 (0.4) |
11.1 (0.2) |
12.0 (0.4) |
64.0 (0.1) |
|
Percentage distribution of enzymes and protein in brown trout liver fractions. B – crude mitochondrial / lysosomal fraction; D – crude peroxisomal fraction; E – crude microsomal fraction; F – cytosolic fraction. Data are a mean from 6 fishes and the coefficients of variation are given in brackets. | ||||
Rocha et al. BMC Biochemistry 2003 4:2 doi:10.1186/1471-2091-4-2 |
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