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Specific and per g enzyme activities in brown trout liver |
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| Enzymes |
Activity per g of liver s-1 g-1 liver (*) or nmol min-1 g-1 liver |
Specific activity s-1 mg-1 protein (*) or nmol min-1 mg-1 protein |
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| A |
B |
D |
E |
F |
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| Peroxisomal markers |
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| Catalase (*) |
95.9 (0.4) |
1.3 (0.2) |
2.0 (0.3) |
3.5 (0.3) |
0.4 (0.3) |
0.6 (0.2) |
| Urate oxidase |
790.4 (0.6) |
10.3 (0.6) |
13.0 (0.7) |
22.6 (0.6) |
4.7 (0.8) |
8.8 (0.8) |
| D-aminoacid oxidase |
471.0 (0.6) |
6.0 (0.4) |
8.9 (0.5) |
14.4 (0.1) |
1.4 (0.4) |
3.5 (0.4) |
| Fatty acyl-CoA oxidase |
73.3 (0.5) |
1.0 (0.4) |
0.4 (0.5) |
1.5 (0.3) |
0.3 (0.8) |
1.0 (0.5) |
| Glycolate oxidase |
60.2 (0.5) |
0.8 (0.3) |
0.8 (0.4) |
2.3 (0.4) |
0.5 (0.5) |
0.7 (0.4) |
| Mitochondrial marker |
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| Succinate dehydrogenase |
310.7 (0.5) |
3.6 (0.3) |
29.1 (0.5) |
27.8 (0.5) |
1.4 (0.7) |
0.2 (0.6) |
| Lysosomal marker |
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| Aryl sulphatase |
275.0 (0.2) |
3.2 (0.4) |
10.4 (0.5) |
10.6 (0.5) |
2.4 (0.4) |
1.4 (0.4) |
| Microsomal marker |
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| NADPH cytochrome c reductase |
2520 (0.4) |
32.5 (0.3) |
29.2 (0.4) |
41.8 (0.3) |
99.0 (0.5) |
11.5 (0.3) |
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| Protein per g of liver (mg g-1 liver) |
Total protein per fraction (mg) |
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| 77.8 (0.2) |
225.6 (0.2) |
26.4 (0.5) |
22.3 (0.3) |
25.4 (0.6) |
128.4 (0.2) |
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Activities per g of liver and specific activities were measured at 37°C in liver homogenates and cell fractions. Protein per mg of liver and in cell fractions is also given. A – liver homogenates; B – crude mitochondrial/lysosomal fraction; D – crude peroxisomal fraction; E – crude microsomal fraction; F – cytosolic fraction. Presented data are a mean from 6 fishes and the coefficient of variation is given in brackets. (*) – Catalase activity is expressed by the first-order rate constant (s-1) and all other enzyme activities are express in nmol min-1, per mg of protein or per g of liver. | ||||||
Rocha et al. BMC Biochemistry 2003 4:2 doi:10.1186/1471-2091-4-2 |
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