Figure 10.

¹⁹F NMR spectra of 6-trifluoromethyl-7-oxo-8-ribityllumazine (Compound 7) in titration experiments with C48A mutant of riboflavin synthase of S. pombe. The initial concentration of the protein was 0.24 mM. Compound 7 was added to the concentrations indicated in the spectra. The spectra are displayed with equal intensities of the signals as indicated by *. For other details see legend to Fig. 5.