Table 3

Variation of BDH kinetic parameters (K_M and K_D) for BOH, NAD⁺, AcAc and NADH calculated from brain and liver enzymes activities under different physiological conditions. Values are given in mean ± standard deviation of at least two experiments with 4 different animals for each condition at *P < 0.05 as compared to the active (A) or at **P < 0.05 as compared to the prehibernating (PH) calculated by ANOVA test.

BOH

NAD⁺

AcAc

NADH, H⁺

Tissue

Physiological states

K_M (mM)

K_M (mM)

K_D (mM)

K_M (mM)

K_M (mM)

K_D (mM)

Liver

Euthermic(active)

0.633 ± 0.05

0.238 ± 0.015

2 ± 0.18

0.150 ± 0.01

0.074 ± 0.005

0.15 ± 0.01

Prehibernating

1.06 ± 0.1 (× 1.67)*

0.55 ± 0.04 (× 2.3)*

1.33 ± 0.1 (× 6.8)*

1.026 ±

0.008 (× 6.8)*

0.28 ±

0.018 (× 3.8)*

0.4 ± 0.03 (× 2.66)*

Hibernating

0.944 ± 0.07

(× 1.49)*

0.174 ± 0.014

(× 0.73)*

0.444 ± 0.035

(× 0.22)*

0.146 ± 0.01

(× 0.97)

0.058 ± 0.005

(× 0.78)*

0.12 ± 0.01 (× 0.8)*

Brain

Euthermic(active)

2 ± 0.15

1.187 ± 0.1

1.9 ± 0.1

0.216 ± 0.018

0.076 ± 0.005

0.16 ± 0.01

Prehibernating

2.25 ± 0.2

(× 1.25)

1.35 ± 0.1

(× 1.13)

1.72 ± 0.1

(× 0.9)

0.287 ± 0.02

(× 1.33)*

0.066 ± 0.004

(× 0.87)*

0.2 ± 0.0175 (× 1.25)*

Hibernating

1.33 ± 0.12

(× 0.66)*

0.787 ± 0.06

(× 0.66)*

3.11 ± 0.25 (× 1.64)*

0.067 ± 0.004

(× 0.30)*

0.035 ± 0.002

(× 0.46)*

0.9 ± 0.06

(× 5.62)*

Kabine et al. BMC Biochemistry 2003 4:11   doi:10.1186/1471-2091-4-11