Figure 9.

Site-directed change of ser11 and ser25 to glutamic acid prevents nuclear entry of annexin II. GFP-fused annexin II containing Ser to Ala or Ser to Glu either singly or in combination were generated as described in materials and methods. Transient transfection of LNCaP cells was performed followed by treatment with or without LMB. Confocal microscopic observations were made and representative images are shown. Bar: 10 μm.