|
Kinetic constants for the high affinity component of ThDP uptake by mitochondria isolated from various cell types. |
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| cell line |
Km |
Vmax |
|
|
||
| normal lymphoblasts |
0.38 |
2.0 |
| TRMA lymphoblasts |
0.60 |
1.5 |
| fibroblasts |
0.41 |
10 |
| neuroblastoma |
0.20 |
39 |
| glyB |
0.41 |
0.8 |
|
Km, micromolar; Vmax, pmol per mg protein per min. Values for normal lymphoblasts are derived from a Lineweaver-Burk plot using the mean velocity values averaged from 4 independent experiments. Two independent experiments were performed for TRMA lymphoblasts, fibroblasts, and neuroblastomas, and one experiment was performed for glyB cells. | ||
Song and Singleton BMC Biochemistry 2002 3:8 doi:10.1186/1471-2091-3-8 |
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