Table 1

Kinetic parameters for the hydrolysis of CoA and CoA derivatives by Trx-Y87G2A.14 fusion protein

Substrate

Km (mM)

kcat (s-1)

kcat / Km (103M-1.s-1)


CoA

0.22

13.8

64

Acetyl-CoA

0.23

11.8

51

Succinyl-CoA

0.24

12.7

53

3-Hydroxymethylglutaryl-CoA

0.20

10.5

54

CoASSCoA

0.32

2.9

9.1


Data were collected using the HPLC assay described in Materials and methods.

AbdelRaheim and McLennan BMC Biochemistry 2002 3:5   doi:10.1186/1471-2091-3-5

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