|
Steady-state kinetic constants for hydrolysis of penicillins by wild-type L1 and L1 mutants. |
||||||
| pencilin G |
ampicillin |
|||||
| Enzyme |
Km (μM) |
kcat (s-1) |
kcat/Km × 107 |
Km (μM) |
kcat (s-1) |
kcat/Km × 107 |
|
|
||||||
| w.t. |
38 ± 12 |
600 ± 100 |
1.6 |
55 ± 5 |
520 ± 10 |
0.95 |
| S224A |
70 ± 20 |
580 ± 100 |
0.83 |
125 ± 13 |
339 ± 1 |
0.27 |
| S224K |
44 ± 8 |
124 ± 12 |
0.28 |
25 ± 3 |
152 ± 2 |
0.61 |
| S224D |
1600 ± 200 |
42 ± 9 |
0.0026 |
1100 ± 240 |
10 ± 1 |
0.00091 |
| I164A |
60 ± 5 |
698 ± 100 |
1.2 |
43 ± 3 |
524 ± 100 |
1.2 |
| F158A |
50 ± 5 |
138 ± 10 |
0.28 |
165 ± 20 |
270 ± 30 |
0.16 |
| Y228A |
410 ± 60 |
609 ± 64 |
0.15 |
710 ± 74 |
443 ± 10 |
0.062 |
| Y228F |
140 ± 14 |
630 ± 30 |
0.45 |
271 ± 40 |
243 ± 30 |
0.090 |
| N233L |
33 ± 9 |
184 ± 35 |
0.56 |
90 ± 20 |
508 ± 40 |
0.56 |
| N233D |
60 ± 2 |
440 ± 86 |
0.73 |
117 ± 18 |
621 ± 31 |
0.53 |
Carenbauer et al. BMC Biochemistry 2002 3:4 doi:10.1186/1471-2091-3-4 |
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