|
Summary of the purification protocol of the protease produced by Trichoderma harzianum 1051. |
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| Purification Step |
Total activity (U) |
Total protein (mg) |
Specific activity (U/mg) |
Purification (fold) |
Yield(%) |
|
|
|||||
| *Freeze-dryed culture medium |
274 |
5.0 |
55 |
- |
100 |
| ** crude enzyme |
220 |
7.5 |
29.3 |
1 |
80 |
| ***phenyl-Sepharose eluate |
75 |
0.63 |
119 |
4.06 |
27 |
|
*Freeze-dried culture medium ressuspended with 20 ml of 50 mM phosphate buffer, pH 5.0; **pellet resulting from precipitation of the freeze-dried material, with 60% (NH4)2 SO4 and resuspended with 3 ml of the phosphate buffer; *** Protease sample (Fig. 1, fractions 30–37) resulting from chromatography of the crude enzyme on a phenyl-Sepharose column. | |||||
De Marco and Felix BMC Biochemistry 2002 3:3 doi:10.1186/1471-2091-3-3 |
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