Figure 5.

Kinetics of association of TFO2 to DS2 A) Example of the association kinetics. DS2 (0.5 μM) and TFO2 (2.5 μM) were mixed in binding buffer and incubated at 37°C in the cuvette. The fluorescence emission spectra were recorded at various time points. B) The TAMRA fluorescence of the spectra shown in A was determined and the data fitted to an equation describing the kinetics of a bimolecular binding reaction.