Figure 4.

Equilibrium binding constant of TFO2 to DS2 A) To measure the binding affinity of TFO2 to DS2, DS2 (0.5 μM) was incubated with different amounts of TFO2 (10 nM to 10 μM) in binding buffer for 10 hours and the fluorescence spectra recorded. B) The TAMRA fluorescence was determined and fitted to an equation describing a bimolecular binding equilibrium.