Figure 3.

Dependence of triplex formation on divalent cations To analyze the dependence of triplex formation on divalent cations, DS1 (0.5 μM) was annealed to TFO (10 μM) in binding buffer (20 mM HEPES pH 7.5, 50 mM Na-acetate, 10 mM MgCl₂) and in buffer not containing Mg²⁺-ions (20 mM HEPES pH 7.5, 50 mM Na-acetate). In the Mg²⁺ free buffer, no triplex formation is detectable.