Table 3 |
||||
|
Kinetic parameters of hAps1, hAps1-39N and hAps2 |
||||
|
Enzyme |
Substrate |
Km (μM) |
kcat (s-1) |
kcat/Km (103M-1s-1) |
|
|
||||
|
hAps1 |
Ap6A |
13 ± 5 |
0.17 ± 0.04 |
13.1 |
|
Ap5A |
37 ± 9 |
0.4 ± 0.1 |
10.8 |
|
|
PP-InsP5 (n = 2) |
4 |
0.20 |
50 |
|
|
hAps1-39N |
Ap6A |
11 ± 3 |
0.16 ± 0.02 |
14.5 |
|
Ap5A |
50 ± 10 |
0.4 ± 0.1 |
8.0 |
|
|
PP-InsP5 (n = 2) |
0.3 |
0.09 |
290 |
|
|
hAps2 |
Ap6A |
19 ± 1 |
0.20 ± 0.01 |
10.5 |
|
Ap5A |
50 ± 10 |
0.8 ± 0.2 |
16.0 |
|
|
PP-InsP5 |
1.3 ± 0.6 |
0.14 ± 0.02 |
110 |
|
|
|
||||
|
Leslie et al. BMC Biochemistry 2002 3:20 doi:10.1186/1471-2091-3-20 |
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