Table 3

Kinetic parameters of hAps1, hAps1-39N and hAps2

Enzyme

Substrate

Km (μM)

kcat (s-1)

kcat/Km (103M-1s-1)


hAps1

Ap6A

13 ± 5

0.17 ± 0.04

13.1

Ap5A

37 ± 9

0.4 ± 0.1

10.8

PP-InsP5 (n = 2)

4

0.20

50

hAps1-39N

Ap6A

11 ± 3

0.16 ± 0.02

14.5

Ap5A

50 ± 10

0.4 ± 0.1

8.0

PP-InsP5 (n = 2)

0.3

0.09

290

hAps2

Ap6A

19 ± 1

0.20 ± 0.01

10.5

Ap5A

50 ± 10

0.8 ± 0.2

16.0

PP-InsP5

1.3 ± 0.6

0.14 ± 0.02

110


Leslie et al. BMC Biochemistry 2002 3:20   doi:10.1186/1471-2091-3-20

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