Figure 1.

Gene expression analysis of uninfected and HIV-1 infected cells. A) Both CEM (uninfected) and ACH₂ (latently HIV-1 infected) cells were grown to mid-log phase of growth and processed for RNA isolation. Total RNA was labeled with ³²P-ATP and hybridized to human cDNA filters (Clontech, 588 genes). Blots were hybridized overnight, washed the next day, and exposed to a PhosphorImager cassette. B) Same as in panel A, except all the 588 genes were plotted as fold change vs. gene index (individual genes). Examples of three genes such as prothymosin-α, C-myc, and p21/Waf1, is shown on the diagram. C) Northern blot analysis of prothymosin-α, C-myc, p21/Waf1 and ubiquitin using 10 μg of total RNA, separated on 0.8% formaldehyde gel, and probed with 40 mer anti-sense oligos against respective genes. Bottom of panel C, last insert shows RNA ethidium bromide stain from CEM and ACH₂ cells.