Figure 5.

Long incubations with Rapamycin can alter the growth of some mycobacterial species. (A) Fast-growing M. smegmatis was grown in 7H9 + OADC in the presence of either DMSO (control) or Rapamycin (10 uM). Shown is the OD600 (+/− SEM) at the time intervals described of 7 replicate cultures from 2 independent experiments. The last time point for each growth curve comparing DMSO treatment versus Rapamycin treatment was compared by Student’s T test and found non significant (NS). (B) Slow-growing BCG and M. kanasii were grown in 7H9 + OADC in the presence of either DMSO (control) or Rapamycin (10 uM). Shown is the OD600 (+/− SEM) at the time intervals described of 10 replicate cultures from 2–3 independent experiments per species. The last time points for each growth curve comparing DMSO treatment versus Rapamycin treatment were compared by Student’s T test. Asterisks indicate p < 0.05. (C) The indicated pathogenic mycobacterial species were grown in 7H9-OADC in the presence of DMSO (control) or Rapamycin (10 ug/ml). A laboratory strain (H37Rv), KZN drug-sensitive strain (V9124 [S]), a multidrug resistant (MDR) strain (V2475 [M]), and an extensively drug resistant (XDR) strain (TF275 [X]) were used. Shown is the OD600 (+/− SEM) at the time intervals described of 7–8 replicates from 2 independent experiments. The last time points for each growth curve comparing DMSO treatment versus Rapamycin treatment were compared by Student’s T test. Asterisks indicate p < 0.05.

Zullo et al. BMC Biochemistry 2014 15:4   doi:10.1186/1471-2091-15-4
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