Senorgrams from surface plasmon resonance (SPR) experiments. Biotinylated HCII 1-75 was bound to streptavidin-coated sensor chips and varying concentrations of thrombin were flowed over the chip, generating sensorgrams (e.g. Panel A) of the response in response units (RU) versus the time in seconds. Panel B is a plot of experiment shown in Panel A, combined with two other independent experiments; each point therefore represents the mean mid-plateau response ± the SD of 3 independent experiments. In panels C-F the thrombin concentration was fixed at 250 nM and competitor peptides identified in each panel were combined with thrombin and flowed over the chip. The concentration of competitor peptides in each experiment is shown to the left of the sensorgram.
Boyle et al. BMC Biochemistry 2013 14:6 doi:10.1186/1471-2091-14-6