Peptide phosphorylation by purified RTKB2 kinase. The enzymatic activity of RTKB2 (1 μM) towards four synthetic peptides (750 μM) was measured using the phosphocellulose paper binding assay. Reactions were carried out for 15 minutes at 30°C. Error bars indicate standard deviations.
Schultheiss et al. BMC Biochemistry 2013 14:4 doi:10.1186/1471-2091-14-4