Research article
Metazoan-like signaling in a unicellular receptor tyrosine kinase
1 Department of Physiology and Biophysics, Basic Science Tower, T-6, School of Medicine, Stony Brook University, Stony Brook, NY, 11794-8661, USA
2 Pharmacology, School of Medicine, Stony Brook University, Stony Brook, NY, 11794-8661, USA
BMC Biochemistry 2013, 14:4 doi:10.1186/1471-2091-14-4
Published: 12 February 2013Additional files
Additional file 1: Figure S1:
SDS-PAGE analysis of RTKB2 kinase domain. Lanes 1, 2, and 3 show 0.3, 1.5, and 4.0 μg of purified RTKB2 kinase. Detection: Coomassie blue staining.
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Additional file 2: Figure S2:
(A) SDS-PAGE of purified RM2-6 (Coomassie staining). (B) MALDI-MS analysis of RM2-6 domain.
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Additional file 3: Figure S3:
Immunofluorescence microscopy of SYF cells expressing GFP (top) or GFP-RTKB2-cyto (bottom).
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Additional file 4: Figure S4:
1H 15N HSQC spectrum of the RM2-6 domain from Monosiga brevicollis RTKB2 kinase. The spectrum indicates that RM2-6 is disordered because its backbone amide resonances exhibit a narrow and clustered chemical shift environment that is typical of an unfolded protein.
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