Open Access Highly Accessed Research article

Metazoan-like signaling in a unicellular receptor tyrosine kinase

Kira P Schultheiss1, Barbara P Craddock1, Michael Tong2, Markus Seeliger2 and W Todd Miller1*

Author Affiliations

1 Department of Physiology and Biophysics, Basic Science Tower, T-6, School of Medicine, Stony Brook University, Stony Brook, NY, 11794-8661, USA

2 Pharmacology, School of Medicine, Stony Brook University, Stony Brook, NY, 11794-8661, USA

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BMC Biochemistry 2013, 14:4  doi:10.1186/1471-2091-14-4

Published: 12 February 2013

Additional files

Additional file 1: Figure S1:

SDS-PAGE analysis of RTKB2 kinase domain. Lanes 1, 2, and 3 show 0.3, 1.5, and 4.0 μg of purified RTKB2 kinase. Detection: Coomassie blue staining.

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Additional file 2: Figure S2:

(A) SDS-PAGE of purified RM2-6 (Coomassie staining). (B) MALDI-MS analysis of RM2-6 domain.

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Additional file 3: Figure S3:

Immunofluorescence microscopy of SYF cells expressing GFP (top) or GFP-RTKB2-cyto (bottom).

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Additional file 4: Figure S4:

1H 15N HSQC spectrum of the RM2-6 domain from Monosiga brevicollis RTKB2 kinase. The spectrum indicates that RM2-6 is disordered because its backbone amide resonances exhibit a narrow and clustered chemical shift environment that is typical of an unfolded protein.

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