Figure 5.

Thermal unfolding curves of wild type or mutant chitosanases in the absence or presence of chitosan. (A) wild type. (B) R42K chitosanase. (C) R42E chitosanase. (a) no chitosan. (b) 82.5% DDA chitosan. (c) 99% DDA chitosan. The unfolding process was monitored by intrinsic tryptophan fluorescence.

Lacombe-Harvey et al. BMC Biochemistry 2013 14:23   doi:10.1186/1471-2091-14-23
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