Open Access Highly Accessed Research article

Hepatoma-derived growth factor and nucleolin exist in the same ribonucleoprotein complex

Stephanie Bremer1, Katharina Klein1, Angela Sedlmaier1, Mekky Abouzied2, Volkmar Gieselmann1 and Sebastian Franken13*

Author Affiliations

1 Institute of Biochemistry and Molecular Biology, University of Bonn, Nussallee 11, Bonn, 53115, Germany

2 Faculty of Pharmacy, University of El-Minia, El-Minia, Egypt

3 Present address: Life-Science-Inkubator, Ludwig-Erhard-Allee 2, Bonn, 53175, Germany

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BMC Biochemistry 2013, 14:2  doi:10.1186/1471-2091-14-2

Published: 10 January 2013

Additional files

Additional file 1:

HDGF interactome. Four biological replicates each were analyzed for HDGFStrep-tag or GFPStrep-tag copurifying proteins by ESI-MS/MS. Proteome discoverer software were used to produce multiconsensus lists of the four HDGF and the four GFP replicates. Lists were filtered for proteins with at least 2 peptide hits and at least 10 peptide spectrum matches (PSM). Afterwards proteins present in both lists were subtracted to receive the HDGF interactome.

Format: XLSX Size: 18KB Download file

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Additional file 2:

Multiconsensus list of four HDGF measurements. Four biological replicates each were analyzed for HDGFStrep-tag copurifying proteins by ESI-MS/MS. Proteome discoverer software were used to produce a multiconsensus list of the four HDGF replicates. This list was filtered for proteins with at least 2 peptide hits and at least 10 peptide spectrum matches (PSM).

Format: XLSX Size: 36KB Download file

Open Data

Additional file 3:

Multiconsensus list of four GFP measurements. Four biological replicates each were analyzed for GFPStrep-tag copurifying proteins by ESI-MS/MS. Proteome discoverer software were used to produce a multiconsensus list of the four GFP replicates. This list was filtered for proteins with at least 2 peptide hits and at least 10 peptide spectrum matches (PSM).

Format: XLSX Size: 30KB Download file

Open Data

Additional file 4:

Gene ontology annotations of the HDGF interactome. Proteins present in the HDGF interactome (see Additional file 1) were analyzed for Biological Process, Cellular Compartment and Molecular Function via the AmiGo online analysis tool (http://amigo.geneontology.org webcite).

Format: XLSX Size: 25KB Download file

Open Data

Additional file 5:

Peptide hits of proteins processed by SDS-PAGE and ESI-MS/MS. ESI-MS/MS files were searched against Swissprot database by the MASCOT search engine. Peptide residues of the identified proteins were listed together with measured and calculated peptide masses.

Format: XLSX Size: 12KB Download file

Open Data

Additional file 6:

Western blot against Ku86/XRCC5. To confirm mass spectrometric results, lysates of HEK293 cells expressing HDGFStrep-tag fusion proteins and their eluate fractions from HDGFStrep-tag purification were examined by Western blot with a specific Ku86 antibody. Ku86 is only present in eluates from cells expressing HDGFStrep-tag fusion proteins.

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