Table 1

List of primers used for mutagenesis
Purpose Primer sequence (5′ to 3′)
saturated mutagenesis of GCCTCAGATAATGATTTTTCCAGAAGGAACTNNKACAAA-
Cys-211 codon TAGGACCTGCCTCA
Cys-211 to Ser GATTTTTCCAGAAGGAACTAGTACAAATAGGACCTGCCT
Glu-403 to Ala TGAGATTGACCTTCGTGCATATGTGGTCGCCTTGT
Cys-216 to Ala GAACTGCTACAAATAGGACCGCCCTCATTACCTTCAAACCTG
Cys-263 to Ala AATCCTGTGGCTCACTCTGGCCCAGTTTCAAAACCAAGTG
Cys-278 to Ala GTGGAAATTGAATTTCTGCCTGTGTATGCCCCTTCTGAAGAGG
Cys-314 to Ala ACTATACATTTGAGGACGCCCAGCTGGCTCTGGCAG
Cys-330 to Ala CTTGCCTGCTGACACCGCCCTGCTAGAGTTTGCC
Cys-401 to Ala GCCTTGTCTGTGGTGGCCAGGCCATCCCAGAC
Cys-443 to Ala GAGGCCAACCTGTCCGCCATCCTCAAGACTGC
Cys-477 to Ala AATCACCTTTGATGACTTCGCCGGGTTTGCGGAAATGTAC
Cys-501 to Ala GACACATTTCGACAGCGCTGCACAGACACCCCCA
Cys-514 to Ala CAACTCCCAATGGCTTCGCCATTGACTTCAGCCCTG
Cys-530 to Ala ACTTTGGGAGAAAGAATTCTGCTAAGAAAGCGGACTAGCCTC

Soupene and Kuypers

Soupene and Kuypers BMC Biochemistry 2012 13:8   doi:10.1186/1471-2091-13-8

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