1D-PAGE (12%) electrophoretic profiles of proteins collected from silkworm B. mori at different developmental stages. The schematic provides a simplified overview of the changes in relative protein concentration as estimated by staining intensity in bands of 29, 30 and 31 kDa; the corresponding original gel data are provided in supplementary files for closer inspection. For detailed explanations see text. 50 μg per lane of protein was loaded. The insert shows an FE-SEM image of native PVFB tissue from 3rd day of V instar; magnification 1.000. V1-V6: Day 1 to day 6 of V instar larval stage; S0-S2: Day 0 to day 2 of spinning stage; P0-P6: Day 0 to day 6 of pupal stage; M: Molecular weight marker. A) PPFB, larval and spinning stages. 30 kDa proteins are synthesized and secreted into hemolymph (see Additional file 2). B) Hemolymph, larval, spinning and pupal stages. 30 kDa protein concentration increases during synthesis and decreases with uptake into PVFB (see Additional file 3). C) PVFB, larval, spinning and pupal stages. 30 kDa protein concentration increases during uptake and storage and decreases during late pupal stages and transformation to adult (see Additional file 4).
Pakkianathan et al. BMC Biochemistry 2012 13:5 doi:10.1186/1471-2091-13-5