K48E and K48F mutants mediate less CB release from liposomes than WT SeHAS. Purified WT (black circles) and K48E (Panel A, black squares) or K48F (Panel B, black squares) SeHAS were added to CB-containing liposomes and FL quenching was monitored at 30°C for the indicated times as in Fig. 2.
Medina et al. BMC Biochemistry 2012 13:2 doi:10.1186/1471-2091-13-2