SeHAS-dependent quenching in extrusion liposomes is concentration dependent. Different amounts of purified SeHAS, in the same volume of Elution Buffer, were added to CB-containing liposomes at 30°C to final concentrations of 50 (black squares), 100 (black triangles), or 200 (black circles) nM and FL changes were recorded as in Fig 2. In this and the following Figs, FL quenching (1.0 - relative FL) is graphed, rather than relative FL. After 1800 seconds, quenching was 3%, 6%, and 14% in response to addition of 50, 100 and 200 nM SeHAS, respectively.
Medina et al. BMC Biochemistry 2012 13:2 doi:10.1186/1471-2091-13-2