Experimental model for monitoring HAS-mediated FL quenching in liposomes. Step 1 in the scheme depicts the addition of purified SeHAS (cylinders) to extrusion liposomes (large circles) containing free CB (small circles). In step 2, HAS molecules insert into the liposome bilayer, allowing the small CB dye to translocate (efflux) through the HAS pores to the exterior if an intraprotein pore is present. In experiments with continuous FL monitoring in the presence of external anti-CB Ab (black Y), CB that effluxes from the lumen is bound by Ab resulting in FL quenching (step 3).
Medina et al. BMC Biochemistry 2012 13:2 doi:10.1186/1471-2091-13-2