Position of the LS substitutions that do not prevent RicA binding to Rab2. The predicted accessible surface of the trimeric structure is depicted in each view. The position of the LS substitutions (score > 1 in the Blosum62 matrix) is indicated with the following color code : the substitutions found in mutated alleles only are shown in green, substitutions found in C. crescentus homolog only are shown in red, residues substituted in the mutated alleles and in the C. crescentus homolog are shown in yellow. In each picture, the ribbon of one monomer (as depicted in Figure 1) is shown by transparency, but in (A) on the right, the transparency is increased to allow the correspondence with Figure 1. (A) Position of the exposed β-sheet and the IGFP loop, in a “side view”. The arbitrary “top” and “bottom” of the structure are indicated. (B) and (C) Top and bottom views of the substitutions on the predicted trimeric structure of RicA, respectively.
Nkengfac et al. BMC Biochemistry 2012 13:16 doi:10.1186/1471-2091-13-16