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The intrinsic GTPase activity of the Gtr1 protein from Saccharomyces cerevisiae

Palanivelu Sengottaiyan1, Cornelia Spetea2, Jens O Lagerstedt3, Dieter Samyn1, Michael Andersson1, Lorena Ruiz-Pavón1 and Bengt L Persson14*

Author Affiliations

1 School of Natural Sciences, Linnaeus University, SE-391 82, Kalmar, Sweden

2 Department of Biological and Environmental Sciences, University of Gothenburg, SE-405 30, Gothenburg, Sweden

3 Department of Experimental Medical Science, Lund University, SE-221 84, Lund, Sweden

4 Laboratory of Molecular Cell Biology, Institute of Botany and Microbiology, Katholieke Universiteit Leuven, and Department of Molecular Microbiology, Flanders Institute of Biotechnology (VIB), Kasteelpark Arenberg 31, BE-3001, Leuven-Heverlee, Flanders, Belgium

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BMC Biochemistry 2012, 13:11  doi:10.1186/1471-2091-13-11

Published: 24 June 2012

Additional files

Additional file 1:

Supplemental methods - Expression in Escherichia coliand purification of Gtr1 protein [12].

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Additional file 2:

Figure S1 - Assessment of the oligomeric state of recombinant Gtr1 protein by native gel electrophoresis. Figure S2 - Stability of the nucleotide-bound Gtr1 Cys-less protein assayed by native gel electrophoresis and Western blotting.

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