Oligomerization domain of Bcr mediates cross-linking by TG2. A) Schematic view of Bcr and Abr constructs. O, oligomerization domain; PK, protein serine/threonine kinase domain; DH, Dbl homology domain; PH, Pleckstrin homology domain; C2, domain with homology to C2 domains that are calcium-dependent, protein or phospholipid binding; GAP, GTPase activating protein. B) Xpress-tagged Abr, Bcr wild type (WT), or Bcr mutant lacking the oligomerization domain (Δoligo) were co-transfected with TG2. Lysates were incubated with or without 5 mM CaCl2. C) Lysates of cells co-transfected with TG2 and Xpress-tagged BcrWT or BcrΔoligo were incubated with CaCl2 for the indicated times. Asterisks, as in Figure 1.
Yi et al. BMC Biochemistry 2011 12:8 doi:10.1186/1471-2091-12-8