Table 4

Peak area ratios of the peptide cleavage product divided by the internal standard peptide obtained from the Endopep-MS reaction of BoNT B after its extraction by the antibody panel.

Antibody

BoNT/B1

BoNT/B2

BoNT/B3

BoNT/B4

BoNT/B5

None

1.02

1.62

0.46

0.14

1.26

B11E8

4.03

1.68

5.71

0.002

1.84

1B12.3

3.58

1.68

4.31

0.09

1.85

2B18.2

3.47

1.25

5.58

0.29

1.60

B8.1

3.30

1.44

0.54

0.14

0.009

B12.2

3.30

1.70

4.14

0.15

1.35

2B30

3.21

1.27

4.44

0.002

1.35

B12.1

3.18

1.89

5.51

0.19

1.65

1B18.1

3.14

1.23

4.98

0.19

1.51

2B18.3

3.14

1.17

5.14

0.17

1.39

2B18.1

3.04

1.15

3.94

0.22

1.62

1B12.4

2.92

1.11

3.35

0.13

2.26

2B24

2.76

1.01

2.93

0.19

1.60

2B23

2.68

1.18

3.24

0.11

1.19

2B25.1

2.38

0.72

1.69

0.05

0.69

A12

2.36

1.47

0.99

0.12

0

B6.1

2.35

1.12

3.16

0.15

1.51

4B19.1

2.31

1.25

3.93

0.21

2.11

6A12

1.39

1.09

2.50

0.07

1.18

1B22

0.95

0.72

3.79

0.03

0.95

2B27

0.92

0.56

1.29

0.05

1.12

2B29

0.88

0.80

1.90

0.03

0.87

1B22.4

0.54

0.21

0.32

0.009

0.06

B1.1

0

0

0

0

0.002

1B10.1

0

0

0

0

0.11

Five different subtypes of BoNT/B were used, and they include /B1, /B2, /B3, /B4, and /B5.

Kalb et al. BMC Biochemistry 2011 12:58   doi:10.1186/1471-2091-12-58