Table 3

Peak area ratios of the peptide cleavage product divided by the internal standard peptide obtained from the Endopep-MS reaction of BoNT/B with its peptide substrate in the presence of the antibody panel.

Antibody

BoNT/B1

BoNT/B2

BoNT/B3

BoNT/B4

BoNT/B5

None

0.43

1.65

0.72

0.48

0.73

CR2

0.38

1.59

0.79

0.51

1.08

6A12

0.78

2.16

0.93

0.35

0.87

A12

0.76

2.11

0.83

0.53

0.96

B12.2

0.68

2.24

0.85

0.53

0.89

B8.1

0.66

2.13

0.95

0.48

1.06

B12.1

0.63

1.75

1.03

0.48

1.30

2B18.2

0.62

1.83

0.8

0.56

1.24

2B24

0.57

1.78

0.74

0.53

1.07

B1.1

0.56

2.31

0.81

0.54

1.72

2B18.1

0.55

1.39

0.88

0.61

1.09

1B18.1

0.51

2.22

1.07

0.68

0.45

2B30

0.50

1.84

1.29

0.93

0.58

2B23

0.46

1.45

0.85

0.56

1.32

B11E8

0.44

2.44

0.89

0.51

0.82

2B18.3

0.44

2.16

0.75

0.64

0.76

1B12.4

0.40

1.28

0.89

0.77

1.18

4B19.1

0.38

1.49

0.92

0.71

0.60

1B22.4

0.37

1.58

0.92

0.28

0.80

1B12.3

0.35

1.31

0.68

0.70

1.07

B6.1

0.32

2.08

0.87

0.32

1.49

1B22

0.31

1.64

1.04

0.27

0.71

2B29

0.30

1.75

0.98

0.42

0.65

2B27

0.28

1.01

0.62

0.24

0.72

2B25.1

0.27

1.01

0.87

0.25

0.70

1B10.1

0

0.02

0.19

0.10

0.65

Five different subtypes of BoNT/B were used, and they include /B1, /B2, /B3, /B4, and /B5.

Kalb et al. BMC Biochemistry 2011 12:58   doi:10.1186/1471-2091-12-58