Table 2

Affinities, domain specificity, and epitopes of 24 human mAbs to BoNT/B.

mAb

BoNT/B epitope

KD BoNT/B1 (pM)

KD BoNT/B2 (pM)

KD BoNT/B3 (pM)

KD BoNT/B4 (pM)


6A12

HC epitope 1

5150

NB

NB

NB


B12.1

HC epitope 1

40.21

15.42

52.28

624.50


B12.2

HC epitope 1

346

90.11

NM

NM


1B12.3

HC epitope 1

75.73

57.05

68.1

356.73


1B12.4

HC epitope 1

15.60

27.11

9.39

399


2B30

HC epitope 1

370(scFv)

190(scFv)

NB

NB


B1.1

HC epitope 2

477(scFv)

NB

NB

NB


B8.1

HC epitope 3

46.03

90.12

NB

NM


A12

HC epitope 3

2100

NB

NB

NB


B11E8

HN epitope 4

6.59

18.1

15.60

NB


1B18.1

HN epitope 5

545

818

976

21


2B18.1

HN epitope 5

63

181

91

312


2B18.2

HN epitope 5

30.82

83.75

228(scFv)

526(scFv)


2B18.3

HN epitope 5

17.4

20.33

128

3108


B6.1

LC epitope 6

6.82

9.18

28.5

9.41


2B24

LC epitope 6

7.8

10.38

5.44

5.96


1B10.1

LC epitope 7

0.33

0.35

1206

0.41


2B27

LC epitope 7-8

165.4

560(scFv)

2320(scFv)

77(scFv)


1B22

LC epitope 8-9

336.1

319.3

221.1

128.6


1B22.4

LC epitope 8-9

139(scFv)

110(scFv)

141(scFv)

129(scFv)


2B25.1

LC epitope 8-9

16.69

53.32

9.07

29.53


2B29

LC epitope 9

856.7

1020

1610

1290


2B23

LC epitope 10

38.07

45.63

54.89

48.23


4B19.1

LC epitope 10

176.1

138.5

115.5

194


Affinities were measured for IgG using flow fluorimetry in a KinExA or for yeast displayed scFv using FACS (indicated as scFv after the KD data). Epitope numbers identify mAbs whose binding sites on BoNT/B overlap; mAb with the same epitope number cannot bind BoNT/B simultaneously. "NB" means no binding detected at the highest concentration of toxin (up to 1 μM) tested. "NM" means no measurement was performed for that specific subtype of toxin.

Kalb et al. BMC Biochemistry 2011 12:58   doi:10.1186/1471-2091-12-58

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