Table 2 |
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|
Affinities, domain specificity, and epitopes of 24 human mAbs to BoNT/B. |
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|
mAb |
BoNT/B epitope |
KD BoNT/B1 (pM) |
KD BoNT/B2 (pM) |
KD BoNT/B3 (pM) |
KD BoNT/B4 (pM) |
|
|
|||||
|
6A12 |
HC epitope 1 |
5150 |
NB |
NB |
NB |
|
|
|||||
|
B12.1 |
HC epitope 1 |
40.21 |
15.42 |
52.28 |
624.50 |
|
|
|||||
|
B12.2 |
HC epitope 1 |
346 |
90.11 |
NM |
NM |
|
|
|||||
|
1B12.3 |
HC epitope 1 |
75.73 |
57.05 |
68.1 |
356.73 |
|
|
|||||
|
1B12.4 |
HC epitope 1 |
15.60 |
27.11 |
9.39 |
399 |
|
|
|||||
|
2B30 |
HC epitope 1 |
370(scFv) |
190(scFv) |
NB |
NB |
|
|
|||||
|
B1.1 |
HC epitope 2 |
477(scFv) |
NB |
NB |
NB |
|
|
|||||
|
B8.1 |
HC epitope 3 |
46.03 |
90.12 |
NB |
NM |
|
|
|||||
|
A12 |
HC epitope 3 |
2100 |
NB |
NB |
NB |
|
|
|||||
|
B11E8 |
HN epitope 4 |
6.59 |
18.1 |
15.60 |
NB |
|
|
|||||
|
1B18.1 |
HN epitope 5 |
545 |
818 |
976 |
21 |
|
|
|||||
|
2B18.1 |
HN epitope 5 |
63 |
181 |
91 |
312 |
|
|
|||||
|
2B18.2 |
HN epitope 5 |
30.82 |
83.75 |
228(scFv) |
526(scFv) |
|
|
|||||
|
2B18.3 |
HN epitope 5 |
17.4 |
20.33 |
128 |
3108 |
|
|
|||||
|
B6.1 |
LC epitope 6 |
6.82 |
9.18 |
28.5 |
9.41 |
|
|
|||||
|
2B24 |
LC epitope 6 |
7.8 |
10.38 |
5.44 |
5.96 |
|
|
|||||
|
1B10.1 |
LC epitope 7 |
0.33 |
0.35 |
1206 |
0.41 |
|
|
|||||
|
2B27 |
LC epitope 7-8 |
165.4 |
560(scFv) |
2320(scFv) |
77(scFv) |
|
|
|||||
|
1B22 |
LC epitope 8-9 |
336.1 |
319.3 |
221.1 |
128.6 |
|
|
|||||
|
1B22.4 |
LC epitope 8-9 |
139(scFv) |
110(scFv) |
141(scFv) |
129(scFv) |
|
|
|||||
|
2B25.1 |
LC epitope 8-9 |
16.69 |
53.32 |
9.07 |
29.53 |
|
|
|||||
|
2B29 |
LC epitope 9 |
856.7 |
1020 |
1610 |
1290 |
|
|
|||||
|
2B23 |
LC epitope 10 |
38.07 |
45.63 |
54.89 |
48.23 |
|
|
|||||
|
4B19.1 |
LC epitope 10 |
176.1 |
138.5 |
115.5 |
194 |
|
|
|||||
|
Affinities were measured for IgG using flow fluorimetry in a KinExA or for yeast displayed scFv using FACS (indicated as scFv after the KD data). Epitope numbers identify mAbs whose binding sites on BoNT/B overlap; mAb with the same epitope number cannot bind BoNT/B simultaneously. "NB" means no binding detected at the highest concentration of toxin (up to 1 μM) tested. "NM" means no measurement was performed for that specific subtype of toxin. |
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|
Kalb et al. BMC Biochemistry 2011 12:58 doi:10.1186/1471-2091-12-58 |
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