Table 1

Kinetic parameter values for 2-oxoglutarate dehydrogenase complex

Basic kinetic parameters

Kinetic parameters for regulatory cofactors

Parameter:

Value

Sensitivity

Parameter:

Value

Sensitivity


Vmax μmol mg-1 min-1a

-

-

KaCa (μM)

0.893

0.293

KmA (mM)

0.273

3.601

KiATP (mM)

0.106

0.943

KmB (μM)

6.96

0.934

KiADP (mM)

0.305

2.073

KmC (μM)

98.6

2.839

KaMg (μM)

19.49

0.228

Kia (mM)

75.9

1.981

<a onClick="popup('http://www.biomedcentral.com/1471-2091/12/53/mathml/M28','MathML',630,470);return false;" target="_blank" href="http://www.biomedcentral.com/1471-2091/12/53/mathml/M28">View MathML</a>

0.262

9.648

Kir (mM)

2.4

1.995

αATP

6.694

1.628

Kic (mM)

0.112

3.970

αADP

0.173

4.412

Kiq (μM)

0.218

5.224

αMg

1.00

6.594

KaH (pH)

6.11

3.623

βMg

4.222

4.603


a We cannot estimate the value of parameter Vf, max in the model without knowing the enzyme activity in the experimental setup. The value of Vmax reported in the table is equal X × Vf, max, where X is the enzyme activity. The optimization estimates that Vmax ≈ 2.16 μmol mg-1 min-1 for Figure 2, Vmax ≈ 11.033 unit/mg for Figure 3 and Figure 4, Vmax ≈ 167 nmol mg-1 min-1 for Figure 5.

Qi et al. BMC Biochemistry 2011 12:53   doi:10.1186/1471-2091-12-53

Open Data