Figure 5.

OGDHC activity regulated by magnesium (A) OGDHC activity of isolated enzyme as a function of ADP in the presence of 1.0 mM NAD, 0.25 mM CoASH, and 0.5 mM 2-oxoglurate with additions of 0.0 (circle), 25 μM (up-triangle), 50 μM (down-triangle) and 200 μM (square) Mg2+. Kinetic data were obtained from Figure Two of [37]. (B-C) OGDHC activity as a function of 2-oxoglutarate in the presence of 1.0 mM NAD, 0.25 mM CoASH with 600 μM (B) or 0 μM (C) Mg2+ addition. (up-triangle) no further additions (control); (circle) +600 μM ATP; (down-triangle) +600 μM ADP. Kinetic data were obtained from Figure Four of [37]. Vmax has been changed less than 10% to better fit the symbols. Solid lines are model fitting results for the data points represented by sym-bols. The experimental data were obtained at pH 7.35 and 30°C in 120 mM KCl, 20 mM MOPS-K, 0.5 mM EGTA buffer.

Qi et al. BMC Biochemistry 2011 12:53   doi:10.1186/1471-2091-12-53
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