Figure 4.

Binding of the MPP8 Chromo domain to H3K9me3 (red) and H3K9me3-S10ph peptides (green) analyzed in solution by fluorescence depolarization. Binding constants were determined by fitting of the data to a binary binding equilibrium to be 0.12 μM for H3K9me3 similarly as observed recently by isothermal calorimetry [13] and > 50 μM for H3K9me3-S10ph.

Bock et al. BMC Biochemistry 2011 12:48   doi:10.1186/1471-2091-12-48
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