PKIα binds almost identical to hCα1, myristylated hCα1, hCα2 and mCα1. GST-PKIα was captured on an α-GST-Antibody sensor chip as described  to surface density of 300 RU. All C-subunits (Cα2, Cα1, myristylated Cα1 and, for comparison, mouse Cα1) were run over the GST-PKIα surface using the assay conditions as described in figure legend 6 in the presence of 1 mM ATP and 5 mM MgCl2. The plot shows the normalized binding data at a concentration of 25 nM C-subunit each.
Vetter et al. BMC Biochemistry 2011 12:40 doi:10.1186/1471-2091-12-40