Figure 3.

Re-oligomerization of adiponectin following diamide treatment. (A) Native PAGE of adiponectin oligomers after treatment with diamide followed by dialysis into PBS. (B) Non-reducing SDS-PAGE analysis of oxidized dimers and reduced monomers at indicated time points. Bovine octadecameric adiponectin was collapsed to trimers as described in Methods. Subsequently, the samples were treated with PBS or 25 mM diamide and allowed to dialyze against PBS for the indicated amount of time. To fix the redox state of adiponectin, NEM was added and stored at 4°C until analysis.