Figure 5.

Secondary structure analysis of CND using CD spectroscopy. (A) CD spectra of tagless CND under various conditions including zinc-bound, after treatment with a chelating agent (apo) and after slowly heating the protein to 90°C (thermally denatured). (B) Thermal denaturation of tagless CND as determined by changes in signal intensity at a wavelength of 222 nm. Fraction folded was calculated as described in Methods.

Arbuckle et al. BMC Biochemistry 2011 12:23   doi:10.1186/1471-2091-12-23
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