Table 3

Effect of reverse transcriptase inhibitors on ORFeus-Mm retrotransposition.

RT inhibitors

Relative retrotransposition efficiencya


5 μM

50 μM

200 μM

400 μM


NRTI

AZT

0.75 ± 0.08

0.16 ± 0.02

d4Tb

0.28 ± 0.05

0.01 ± 0.02

ddC

<0.0025c

<0.0025c

3TC

0.21 ± 0.07

0.08 ± 0.01

ddI

<0.0025c

<0.0025c

ABC

0.80 ± 0.05

0.15 ± 0.03

FTC

0.34 ± 0.01

0.26 ± 0.04

NtRTI

Bis-POM PMPAb

<0.0025c

<0.0025c

NNRTI

NVPb

0.72 ± 0.05

0.66 ± 0.06

1.12 ± 0.08

0.79 ± 0.05

DLVb

0.60 ± 0.05

0.02 ± 0.01


a Transposition efficiency of control assay without inhibitors was considered as 1.0. Transposition efficiency in the presence of inhibitors was indicated as relative efficiency with respect to the control. All data were normalized to cell viability with the treatment of the same amount of inhibitors. Data are mean of six independent experiments ± standard error.

b Transposition efficiency was compared to the control containing the same amount of DMSO

c No colonies when 1×104 cells were plated

d Transposition efficiency could not be detected due to excessive cell death

Dai et al. BMC Biochemistry 2011 12:18   doi:10.1186/1471-2091-12-18

Open Data