Figure 1.

Expression of human L1 reverse transcriptase in E. coli. (A) Domain structure of human L1 ORF2 protein. The conserved domains are shown as shaded rectangles. L1 RT domain (Aa 238-1061) was cloned into pMal-c2X downstream of MBP gene in frame. (B) Human L1 RT domain was expressed in E. coli as a fusion protein (MBP-RT) and purified as described in materials and methods. The sample was resolved by 4-20% SDS/PAGE with Coomassie blue staining. Protein molecular mass markers are to the right (kDa). The arrow indicates the mobility of MBP-RT fusion protein.

Dai et al. BMC Biochemistry 2011 12:18   doi:10.1186/1471-2091-12-18
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