Additional file 2.
Figure S1: Tributyrine plate assay of BSL-B wild type and BSL-B point variants. Tributyrine plate assay of BSL-B wild type as well as BSL-B point variants in which each lysine residue is substituted by alanine and arginine, respectively. -: E. coli BL21(DE3) carrying the empty vector pET19b. WT: E. coli BL21(DE3) expressing BSL-B wild type enzyme (pET19b + lipB). K X A/R: E. coli BL21(DE3) expressing BSL-B in which the lysine residue (K) at position X is substituted with alanine (A) or arginine (R).
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Franken et al. BMC Biochemistry 2011 12:10 doi:10.1186/1471-2091-12-10