Figure 4.

Analysis of glycosylation of LA-a and LA-b. (A) Purified LA-a and LA-b (3 μg per lane) were electrophoresed and stained: SyproRuby for detection of proteins, and Pro-Q emerald 300 for detection of glycoproteins. CandyCane glycoprotein molecular weight standards (0.5 μg for each band) were also loaded. Numbers marked with a star indicate glycoproteins. (B) Purified LA-a and LA-b (1 μg per lane) were electrophoresed, blotted onto PVDF membrane and reacted with lectin RCA120.