Effects of insulin on hepatic glucose output, Akt-phosphorylation and Angptl4 mRNA expression in 12h-fasted male and female rats. (A) Hepatic glucose output was measured in liver perfusates 40 min after saline- or insulin-treatment (n = 4 rats/group). (B) The ratio between phosphorylated (p-Akt-Ser473) and total Akt was determined using ELISA in whole liver cell lysates derived from fasted rats 40 min after saline- or insulin-treatment (n = 6-7 rats/group). (C) Hepatic Angptl4 mRNA levels were quantified by real-time PCR and normalized to the housekeeping gene Arbp (n = 4 rats/group). Data are represented as means ± SEM, and asterisks indicate significant differences compared to corresponding saline-treated group as determined by Student's t-test.
Gustavsson et al. BMC Biochemistry 2010 11:38 doi:10.1186/1471-2091-11-38