Table 1

Relative percent abundance (RPA) data for the mass spectra in Figures 1 and 4

Figure 1a -- Albumin

m/z 66438

m/z 66470

m/z 66557

m/z 66600 & 66614

m/z 66655

m/z 66719

m/z 66818

Sample Treatment

RPA Native

RPA SO₂H

RPA S-Cys

RPA Native Glycated & S-CysGly

RPA NonCov. Phosphate Adduct

RPA S-Cys + Glycation

RPA CPPCHD Adduct

H₂O₂

CPPCHD

32%

10%

38%

6%

3%

5%

6%

+

+

51%

0%

34%

9%

1%

5%

0%

-

+

32%

12%

43%

6%

3%

4%

0%

+

-

49%

0%

36%

6%

3%

5%

0%

-

-

Figure 1b -- TTR

m/z 13761

m/z 13793

m/z 13880

m/z 13937

m/z 14067

m/z 14141

Sample Treatment

RPA Native

RPA SO₂H

RPA S-Cys

RPA S-CysGly

RPA S-Glutathione

RPA CPPCHD Adduct

H₂O₂

CPPCHD

23%

9%

48%

13%

3%

3%

+

+

39%

3%

46%

10%

3%

0%

-

+

21%

12%

52%

12%

3%

0%

+

-

39%

2%

48%

10%

1%

0%

-

-

Figure 4a -- Albumin

m/z 66438

m/z 66470

m/z 66557

m/z 66600 & 66614

m/z 66636

m/z 66655

m/z 66719

Sample Treatment

RPA Native

RPA SO₂H

RPA S-Cys

RPA Native Glycated & S-CysGly

RPA TNB Adduct

RPA NonCov. Phosphate Adduct

RPA S-Cys + Glycation

H₂O₂

TNB

32%

7%

37%

3%

15%

3%

4%

+

+

49%

0%

37%

5%

0%

4%

5%

-

+

32%

13%

42%

5%

0%

3%

4%

+

-

48%

0%

38%

7%

0%

3%

4%

-

-

Figure 4b -- TTR

m/z 13761

m/z 13793

m/z 13880

m/z 13937

m/z 13959

m/z 14067

Sample Treatment

RPA Native

RPA SO₂H

RPA S-Cys

RPA S-CysGly

RPA TNB Adduct

RPA S-Glutathione

H₂O₂

TNB

19%

11%

48%

11%

7%

4%

+

+

29%

IP

50%

9%

8%

4%

-

+

21%

12%

52%

12%

0%

3%

+

-

38%

2%

46%

12%

0%

2%

-

-

IP = "Interfering Peak", cannot properly integrate

Rehder and Borges BMC Biochemistry 2010 11:25   doi:10.1186/1471-2091-11-25