Deletion mapping of PP6c binding to PP6R3. FLAG-tagged full length PP6R3 (lane 1), or residues 1-355 (lane 2), 1-513 (lane 3) or 512-873 (lane 4) were expressed in HEK293 cells and immunoprecipitated with anti-FLAG antibody. FLAG-tagged proteins (upper panel) and co-immunoprecipitated endogenous PP6c (lower panel) were detected by immunoblotting.
Guergnon et al. BMC Biochemistry 2009 10:24 doi:10.1186/1471-2091-10-24